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Researchers Uncover a “Parallel Universe” of Tomato Genetics – Technology Networks

In a new paper appearing in Science Advances, Michigan State University researchers have unraveled a surprising genetic mystery centered on sugars found in what gardeners know as “tomato tar.” 

Anyone who has pruned tomato plants barehanded has likely found their fingers darkened with a sticky, gold-black substance that won’t quite wash off. 

This tomato tar is sticky for good reason. It’s made of sugars — acylsugars, to be precise — and acts as a sort of natural flypaper for would-be pests. 

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“Plants have evolved to make so many amazing poisons and other biologically active compounds,” said Michigan State researcher Robert Last, leader of the new study. 

The Last lab specializes in acylsugars and the tiny, hair-like structures where they’re produced and stored, known as trichomes.  


Once thought to be exclusively found in trichomes, acyl sugars were recently discovered in tomato roots as well by other researchers. This was a surprise for the plant science community.

In its study, the team at MSU wanted to learn how these root acylsugars functioned and just where they came from. 

They found that not only do tomato plants synthesize chemically unique acylsugars in their roots and trichomes, but these acylsugars are produced through two parallel metabolic pathways. 


This is the equivalent of assembly lines in an auto factory making two different models of the same car, but never interacting. 

These discoveries are helping scientists to better understand the resilience and evolutionary story of Solanaceae, or nightshades, a sprawling family of plants that includes tomatoes, eggplants, potatoes, peppers, tobacco and petunias. The findings could also help inform researchers looking to develop molecules made by plants into compounds to help humanity. 

“From pharmaceuticals, to pesticides, to sunscreens, many small molecules that humans have adapted for different uses come from the arms race between plants, microbes and insects,” Last said. 

Roots and shoots 

Beyond key chemicals essential for growth, plants also produce a treasure trove of compounds that play a crucial role in environmental interactions. These can attract useful pollinators and are the first line of defense against harmful organisms.  


“What’s so remarkable about these specialized metabolites is that they’re typically synthesized in highly precise cells and tissues,” said Rachel Kerwin, a postdoctoral researcher at MSU and first author of the latest paper. 

“Take for instance acylsugars. You won’t find them produced in the leaves or stems of a tomato plant. These physically sticky defense metabolites are made right in the tip of the trichomes.” 


When it was reported that acylsugars could be found in tomato roots as well, Kerwin took it as a call for old-fashioned genetic detective work. 


“The presence of these acylsugars in roots was fascinating and led to so many questions. How did this happen, how are they being made and are they different from the trichome acylsugars we’ve been studying?” 

To begin tackling the evolutionary enigma, lab members collaborated with specialists at MSU’s Mass Spectrometry and Metabolomics Core, as well as staff at the Max T. Rogers Nuclear Magnetic Resonance facility. 


In comparing metabolites from tomato seedlings’ roots and shoots, a variety of differences appeared.  


The basic chemical make-up of the aboveground and belowground acylsugars were noticeably different, so much so that they could be defined as different classes of acylsugars entirely. 

Breaking the car 

Last, a University Distinguished Professor in MSU’s College of Natural Science‘s Department of Biochemistry and Molecular Biology and Department of Plant Biology, offers a useful analogy to explain how a geneticist approaches biology. 


“Imagine trying to figure out how a car works by breaking one component at a time,” he said. “If you flatten a car’s tires and notice the engine still runs, you’ve discovered a critical fact even if you don’t know what the tires exactly do.” 

Switch out car parts for genes, and you get a clearer picture of the work accomplished by the Last lab to further crack the code on root acylsugars.

Looking at public genetic sequence data, Kerwin noticed that many of the genes expressed in tomato trichome acylsugar production had close relatives in roots. After identifying an enzyme believed to be the first step in root acylsugar biosynthesis, the researchers began “breaking the car.” 


When they knocked out the root acylsugar candidate gene, root acylsugar production vanished, leaving trichome acylsugar production untouched.  


Meanwhile, when the well-studied trichome acylsugar gene was knocked out, root acylsugar production carried on as usual. 


These findings offered striking proof of a suspected metabolic mirroring.  


“Alongside the aboveground acylsugar pathway we’ve been studying for years, here we find this second parallel universe that exists underground,” Last said. 


“This confirmed we have two pathways co-existing in the same plant,” Kerwin added. 


To drive home this breakthrough, Jaynee Hart, a postdoctoral researcher and second author on the latest paper, looked closer at the functions of trichome and root enzymes. 


Just as trichome enzymes and the acylsugars they produce are a well-studied chemical match, she found a promising link between root enzymes and the root acylsugars as well. 


“Studying isolated enzymes is a powerful tool for ascertaining their activity and drawing conclusions about their functional role inside the plant cell,” Hart explained.  


These findings were further proof of the parallel metabolic pathways that exist in a single tomato plant.  


“Plants and cars are so different, yet similar in that when you open the proverbial hood you become aware of the multitude of parts and connections that make them function. This work gives us new knowledge about one of those parts in tomato plants and prompts further research into its evolution and function and whether we can make use of it in other ways,” said Pankaj Jaiswal, a program director at the U.S. National Science Foundation, which funded the work.  


“The more we learn about living things — from tomatoes and other crops, to animals and microbes — the broader the opportunities to employ that learning to benefit society,” he added. 

Clusters within clusters 

The paper also reports a fascinating and unexpected twist concerned with biosynthetic gene clusters, or BGCs.  


BGCs are collections of genes that are physically grouped on the chromosome and contribute to a particular metabolic pathway.  


Previously, the Last lab identified a BGC containing genes linked to trichome acylsugars in tomato plants. Kerwin, Hart, and their collaborators have now discovered the root-expressed acylsugar enzyme resides in the same cluster.  


“Usually in BGCs, the genes are co-expressed in the same tissues and under similar conditions,” said Kerwin. 


“But here, we have two separate yet interlinked groups of genes. Some expressed in trichomes, and some expressed in roots.” 


This revelation led Kerwin to dive into the evolutionary trajectory of Solanaceae species, with hopes to identify when and how these two unique acylsugar pathways developed.  


Specifically, the researchers drew attention to a moment some 19 million years ago when the enzyme responsible for trichome acylsugars was duplicated. This enzyme would one day be responsible for the newly discovered root-expressed acylsugar pathway. 


The exact mechanism that “switched on” this enzyme in roots remains unknown, paving the way for the Last lab to continue to unpack the evolutionary and metabolic secrets of the nightshade family. 


“Working with Solanaceae provides so many scientific resources, as well as a strong community of researchers,” said Kerwin. 


“Through their importance as crops and in horticulture, these are plants humans have cared about for thousands of years.” 

For Last, these breakthroughs are also a reminder of the importance of natural pesticides, which defense metabolites such as acylsugars ultimately represent.  


“If we find that these root acylsugars are effective at repelling harmful organisms, could they be bred into other nightshades, thereby helping plants grow without the need for harmful synthetic fungicides and pesticides?” Last asked. 


“These are questions at the core of humanity’s pursuit of purer water, safer food and a reduced reliance on harmful synthetic chemicals.” 

Reference: Kerwin RE, Hart JE, Fiesel PD, et al. Tomato root specialized metabolites evolved through gene duplication and regulatory divergence within a biosynthetic gene cluster. Sci Adv. 2024;10(17):eadn3991. doi: 10.1126/sciadv.adn3991

This article has been republished from the following materials. Note: material may have been edited for length and content. For further information, please contact the cited source. Our press release publishing policy can be accessed here.

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Scientists’ research answers big question about our system’s largest planet – Phys.org

Scientists’ research answers big question about our system’s largest planet

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UAF scientist's research answers big question about our system's largest planet
The aurora was photographed in 2014 during a series of Hubble Space Telescope Imaging Spectrograph far-ultraviolet-light observations taking place as NASA’s Juno spacecraft approached and entered into orbit around Jupiter. Credit: NASA, ESA and J. Nichols, University of Leicester

New discoveries about Jupiter could lead to a better understanding of Earth’s own space environment and influence a long-running scientific debate about the solar system’s largest planet.

“By exploring a larger space such as Jupiter, we can better understand the fundamental physics governing Earth’s magnetosphere and thereby improve our space weather forecasting,” said Peter Delamere, a professor at the UAF Geophysical Institute and the UAF College of Natural Science and Mathematics.

“We are one big space weather event from losing communication satellites, our power grid assets, or both,” he said.

Space weather refers to disturbances in the Earth’s magnetosphere caused by interactions between the and the Earth’s magnetic field. These are generally associated with solar storms and the sun’s coronal mass ejections, which can lead to magnetic fluctuations and disruptions in power grids, pipelines and communication systems.

Delamere and a team of co-authors detailed their findings about Jupiter’s magnetosphere in a paper in AGU Advances. Geophysical Institute research associate professor Peter Damiano, UAF graduate student researchers Austin Smith and Chynna Spitler, and former student Blake Mino are among the co-authors.

Delamere’s research shows that our solar system’s largest planet has a magnetosphere consisting of largely closed magnetic field lines at its polar regions but including a crescent-shaped area of open field lines. The magnetosphere is the shield that some planets have that deflects much of the solar wind.

The debate over open versus closed at the poles has raged for more than 40 years.

An open magnetosphere refers to a planet having some open-ended magnetic field lines near its poles. These are previously closed lines that have been broken apart by the solar wind and left to extend into space without re-entering the planet.

This creates regions on Jupiter where the solar wind, which carries some of the sun’s magnetic field lines, directly interacts with the planet’s ionosphere and atmosphere.

Solar particles moving toward a planet on open field lines do not cause the aurora, which largely occurs on closed field lines. However, the energy and momentum of solar wind particles on open field lines does transfer to the closed system.

Earth has a largely open magnetosphere at its poles, with aurora occurring on closed field lines.. It is the transferred energy on those open lines that can disrupt power grids and communications.

In order to study Jupiter’s magnetosphere, Delamere ran a variety of models using data acquired by the NASA Juno spacecraft, which entered Jupiter’s orbit in 2016 and has an elliptical polar orbit.

“We never had data from the polar regions, so Juno has been transformative in terms of the planet’s auroral physics and helping further the discussion about its magnetic field lines,” Delamere said.

Scientist's research answers big question about our system's largest planet
A close-up of Jupiter’s aurora shows auroral footprints of three moons: Io (along the left-hand limb), Ganymede (near the center) and Europa (just below and to the right of Ganymede’s footprint). These emissions flow on Jupiter’s magnetic field. Credit: NASA image, John Clarke, University of Michigan

The debate began with the 1979 flybys of Jupiter by NASA’s Voyager 1 and Voyager 2. That data led many to believe that the planet had a generally open magnetosphere at its poles.

Other scientists argued that Jupiter’s auroral activity, which is much different from Earth’s, indicated the planet had a mostly closed magnetosphere at the poles. Delamere, a longtime researcher of Jupiter’s magnetic field, published a paper supporting that view in 2010.

In 2021, he was a co-author on a paper by Binzheng Zhang of the University of Hong Kong that suggested through modeling that Jupiter’s magnetosphere had two regions of open magnetic field lines at its poles.

The model shows one set of open-ended field lines emerging from the poles and trailing outward behind the planet in the magnetotail, the narrow teardrop-shaped portion of the pointing away from the sun. The other set emerges from Jupiter’s poles and goes off to the sides into space, carried by the solar wind.

“The Zhang result provided a plausible explanation for the open field line regions,” Delamere said. “And this year we provided the compelling evidence in the Juno data to support the model result.

“It is a major validation of the Zhang paper,” he said.

Delamere said it’s important to study Jupiter to better understand Earth.

“In the big picture, Jupiter and Earth represent opposite ends of the spectrum—open versus closed field lines,” he said. “To fully understand magnetospheric physics, we need to understand both limits.”

Delamere’s evidence came via an instrument on the Juno spacecraft that revealed a polar area where ions flowed in a direction opposite Jupiter’s rotation.

Subsequent modeling showed a similar ion flow in the same area—and near the open field lines proposed in the 2021 paper by Zhang and Delamere.

“The ionized gas on [closed] magnetic field lines connected to Jupiter’s northern and southern hemispheres rotates with the planet,” Delamere’s new paper concludes, “while ionized gas on [open] field lines that connect to the solar wind move with the solar wind.”

Delamere writes that the polar location of open “may represent a characteristic feature of rotating giant magnetospheres for future exploration.”

Other contributors are from the University of Colorado Boulder, Johns Hopkins University, Andrews University, Embry-Riddle Aeronautical University, University of Hong Kong, University of Texas San Antonio, Southwest Research Institute and O.J. Brambles Consulting in the United Kingdom.

Delamere will present the research in July at the Conference on Magnetospheres of the Outer Planets at the University of Minnesota.

More information:
P. A. Delamere et al, Signatures of Open Magnetic Flux in Jupiter’s Dawnside Magnetotail, AGU Advances (2024). DOI: 10.1029/2023AV001111

Citation:
Scientists’ research answers big question about our system’s largest planet (2024, May 6)
retrieved 6 May 2024
from https://phys.org/news/2024-05-scientists-big-largest-planet.html

This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no
part may be reproduced without the written permission. The content is provided for information purposes only.


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Ecce Homo: Painting once up for auction for €1,500 confirmed as rare Caravaggio work – Sky News

A painting that had been up for auction for just €1,500 (£1,285) has been confirmed as a lost work of Italian master Michaelangelo Merisi da Caravaggio.

Spain‘s Prado Museum says the work titled Ecce Homo – which means behold the man in Latin – was due to be auctioned in April 2021 as a painting by 17th-century Spanish artist Jose de Ribera.

But before it could go under the hammer at a Madrid auction house, Spanish authorities imposed an export ban on the painting after the museum alerted the government it could be a Caravaggio.

The painting is one of only 60 Caravaggio paintings known to exist, the museum said, and its true value could stretch into tens of millions of euros.

It dates back to 1605-09 and is believed to have once been part of the private collection of Spanish king Phillip IV of Spain, the Prado added, and it will go on display from 27 May until October.

“For our part, we are more than happy to be the stage to present this new unshown work of Caravaggio to the public and critics,” Prado Museum director Miguel Falomir said.

He added since the 19th century it had been in the hands of a family in Madrid, who recently sold it to an individual who wanted it displayed in the Prado Museum and has not been identified.

Read more:
John Lennon’s lost guitar found after 50 years to go up for auction
Acid to destroy Picasso masterpieces if Assange dies in prison, artist claims

The oil-on-canvas work, which measures 111 by 86cm, depicts the Biblical passage of the Ecce Homo, in which Jesus Christ is presented to the crowds before being crucified.

Although now owned by a private individual, the painting will not be allowed to leave Spain without government permission.

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The Prado said the work has been under the custodianship of the Colnaghi art gallery since April 2021.

The painting was restored by specialist Andrea Cipriani and his team under the supervision of experts from the Madrid regional government.

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ZESA Pays US$10 000 In Damages To Man Electrocuted By Hanging Electricity Cables – pindula.co.zw

The Zimbabwe Electricity Supply Authority (ZESA) has forked out ZiG132 889, which is about US$10 000, to a 21-year-old man, as compensation for damages, after he was electrocuted 2 years ago and sustained severe electrical burns owing to gross negligence by the State-run power utility.

According to the Zimbabwe Lawyers for Human Rights (ZLHR), Dumisani Chikosi, who resides in Tonhodzai village in Chiadzwa in Manicaland Province, was electrocuted by some electricity cables that were hanging close to the ground in January 2022 while walking home in the company of four other people.

As a result of the electrocution, Chikosi suffered severe burns all over his body and permanent and irreversible injuries.

Chikosi, who was a teenager at the time that he was electrocuted, went on to spend 80 days at Murambi Garden Clinic in Mutare, where he received treatment. Added ZLHR:

While in hospital, ZESA Holdings through its subsidiary, Zimbabwe Electricity Transmission and Distribution Company (ZETDC), paid for his medical expenses although it was not forthcoming with respect to paying him compensation for the damages, he suffered.

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This then compelled Chikosi to engage Peggy Tavagadza and Tatenda Sigauke of the Zimbabwe Lawyers for Human Rights who in December 2022 wrote a letter of demand to ZETDC Eastern Region offices claiming US$20 000 in damages.

In their letter to ZETDC, Tavagadza and Sigauke told the electricity generating company that it was liable for the unfortunate accident, which was caused by negligence on the part of its employees, who neglectfully failed to secure electrical cables in a residential area, thereby endangering people.

The human rights lawyers said in response to the letter, Cell Insurance, who are ZETDC’s insurers offered a settlement of US$10 000, which Chikosi accepted.

However, ZETDC vacillated and failed to honour its obligations in respect of the offer of settlement, which it had made. Said ZLHR:

This prompted Tavagadza and Sigauke to file a summons at Mutare Magistrates Court on 5 April 2024 seeking an order to enforce payment from ZETDC.

While the matter was yet to be set down for hearing in court, ZETDC immediately made a payment amounting to ZiG132 889, which translates to about US$10 000 to Chikosi, as compensation for the damages, which he suffered from the electrocution. Resultantly, Chikosi withdrew the summons after ZETDC paid him compensation.

ZLHR intervened in assisting the minor child as part of its anti-impunity strategies to foster accountability at the country’s supplier of electricity and to deter and discourage acts of human rights violations by state-run institutions.

Over the years, ZLHR has intervened in similar cases of ZESA Holdings’ negligence by suing and obtaining orders for payment of damages on behalf of several victims.

More: Pindula News

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